Longterm longitudinal studies with large populations of individuals with oral. Frontiers salivary biomarkers for oral squamous cell. Beatriz carrionruiz department of stomatology, faculty of dentistry, oral medicine unit, university of. Oral cancer is one of the major global public health problems and is the sixth most common human malignancy. Oral cancer is the sixth most common cancer type in the world, and 90% of it is represented by oral squamous cell carcinoma oscc. However, some issueschallenges have arisen that need to be resolved in order to establish this approach as a reliable, highly sensitive and specific method for clinical use. We subjected our salivary oral cancer biomarkers to a phase ii validation study with an edrnbrl to assess the reproducibility of the oral cancer salivary biomarkers. The aim of this study was to investigate if certain salivary biomarkers could be used for detection of common systemic diseases. An insight on salivary biomarkers for oral cancer abstract oral cancer is one of the major public health concerns with 12% of all types of cancers worldwide. We tested 19 tongue cancer patients, measuring the.
Despite the groundbreaking findings demonstrated, the authors assert that the ongoing strategy for detecting oscc oral visual inspection and biopsy is ineffective, because it has globally yielded high specificity but varied sensitivity. Reliable biomarkers for oral cancer oc remain scarce, and routine tests for the detection of precancerous lesions are not routine in the clinical setting. The aim of this study was to investigate if certain salivary biomarkers could be used for. Salivary biomarkers in psychological stress diagnosis. Salivary biomarkers for detection of oral squamous cell carcinoma. Salivary diagnostics, oral cancer, oral pre cancer, metabolomics. Salivary biomarkers and their efficacies as diagnostic tools for oral squamous cell carcinoma. Salivary biomarkers in oral squamous cell carcinoma an insight.
Salivary biomarkers for oral cancer and precancer screening. Usefulness of salivary biomarkers in oral precancer and cancer alberto rodriguezarchilla, m. So far, more than 100 potential oscc salivary biomarkers have been reported in the literature, based mainly on comparing. Pdf salivary analysis of oral cancer biomarkers researchgate. Toward future clinical and diagnostic utilities janice m. The discovery of these new targets may lead to a simple clinical tool for the noninvasive diagnosis of oral cancer. In particular, we examine the composition and function of the saliva, with special focus on salivary biomarkers. Over the past decade, there has been a dramatic increase in studies evaluating salivary biomarkers of stress.
Salivary biomarkers for oral squamous cell carcinoma. Within the oral cavity, saliva shows great promise as a noninvasive and sensitive marker for many systemic diseases. Nonetheless, emerging barriers are an obstacle to the study of the salivary proteome in an effective way. Prevalidation of salivary biomarkers for oral cancer detection. Among malignancies, oral cancer is the type in which salivary examination shows the enormous benefits due to its direct interaction with the cancer cells. Usefulness of salivary biomarkers in oral precancer and cancer.
In saliva, the tumorspecific dna was positive in 100% of the patients diagnosed with oral cancer, and 4770% of the patients with tumors in other places of the body also carry specific tumor dna markers in the saliva. Salivary biomarkers in oral squamous cell carcinoma an. Patients with oral cancer have elevated levels of certain protease enzymes in their saliva, a finding that could help with screening and diagnosis. Pdf salivary biomarkers for oral cancer and precancer screening.
Sep 29, 2009 salivary testing, a noninvasive alternative to serum testing, can be an effective modality for diagnosis and prognosis predicting of oral cancer as well as for monitoring the patients post. Reported potential salivary biomarkers for oral cancer detection. Oral squamous cell carcinoma oscc is one of the most common deadly diseases with a poor survival rate of 60%. In this chapter, the potential salivary biomarker signatures. Considering previously published results, we can infer that these salivary biomarkers are useful for oral cancer detection regardless of. Considering previously published results, we can infer that these salivary biomarkers are useful for oral cancer detection regardless of ethnicity. So far, more than 100 potential oscc salivary biomarkers have been reported in the literature, based mainly on comparing the levels found in oscc patients to the levels found in nonoscc normal controls see table 1 1862.
Role of salivary biomarkers in the diagnosis of oral cancer jmrps. Potential salivary mrna biomarkers for early detection of. Salivary protease spectrum biomarkers of oral cancer. In a recently published study, approximately 50 mirnas were detected in saliva using reverse transcriptasepreamplificationquantitative sensitivity %specificity %auc polymerase chain reaction. Clinical significance of salivary biomarkers in oral squamous cell. Over the past two decades, using salivary biomarkers specifically for early cancer detection has attracted much research interest, especially for cancers occurring in the oral cavity and oropharynx, for which the fiveyear survival rate 62% is still one of the lowest among all major human cancers. Salivary testing, a noninvasive alternative to serum testing, can be an effective modality for diagnosis and prognosis predicting of oral cancer as well as for monitoring the patients post. Proteinbased salivary profiles as novel biomarkers for oral.
Salivary tools for the measurement and investigation of alterations in particular salivary molecules such as. Salivary analysis of oral cancer biomarkers british journal. Search for articles involved the medline, pubmed, and embase. Moreover, the unstable rna is protected in exosomes, allowing their stable detection and easy quantification. Longterm longitudinal studies with large populations of individuals with oral cancer and those who are at high risk of developing oral cancer are needed to validate these potential biomarkers. Therefore, the implementation of newer screening and early detection approaches are of utmost importance which could reduce. Role of salivary biomarkers in the diagnosis of oral cancer. Biomarker, dna, mrna, oral squamous cell carcinoma, saliva decreases to as low as 1550%. More accurate oral cancer screening with fewer salivary.
Oral cancer is one of the most common cancers in the world, representing a serious problem for global health shah and gil, 2009. Numerous studies have described the potential of mirnas as cancer biomarkers for oral cancer 72, 73. Salivary biomarkers represent a promising noninvasive approach for oral cancer detection, and an area of strong research interest. Pdf oral cancer is one of the major global public health problems and is the sixth most common human malignancy. Our results support the idea that salivary diagnostics might be used in the clinical practice.
In addition, chronic periodontitis is a risk indicator for oral cavity andor oropharyngeal cancer 12. Such a choice was taken in order to exclude presence of pathologies in the proximity of the site. Several salivary protein markers such as interleukins 8, 6, 1. Saliva protein biomarkers and oral squamous cell carcinoma pnas. Initially, technical and methodologic information was transferred, via handson training, to the operator in an nciedrnsponsored laboratory. The 90% of oral cancer issues have been increased due to smoking and alcohol consumption all over the world. Salivary tools for the measurement and investigation of alterations in particular salivary molecules such as proteins, genes, enzymes, cytokines, metalloproteinases, cytokeratins, growth factors, etc. Salivary nglycosylation as a biomarker of oral cancer. The identification of potential saliva signatures for early, noninvasive detection of oral. Salivary biomarkers list of high impact articles ppts. In a recently published study, approximately 50 mirnas were detected in saliva using reverse. Salivary biomarkers for diagnosis of systemic diseases and. This has opened a new avenue for salivary biomarker discovery for human diseases.
We subjected our salivary oral cancer biomarkers to a phase ii validation study with an. Role of salivary biomarkers in oral cancer detection. Therefore, the implementation of newer screening and early detection. Noninvasive diagnosis of disease and monitoring of general health herenia p.
Direct contact between saliva and the oral cancer lesion makes measurement of tumour. Pdf role of salivary biomarkers in oral cancer researchgate. More accurate oral cancer screening with fewer salivary biomarkers james michael menke1, md shahidul ahsan2 and suan phaik khoo3 1a. The nciedrn is a dedicated entity committed to make molecular diagnostics for cancer a clinical reality. Apr 18, 2020 salivary biomarkers and their efficacies as diagnostic tools for oral squamous cell carcinoma. With the development of salivaomics, increased numbers of biomarkers that are related to oral and systemic diseases have been identified in the saliva.
This study addresses a current unmet need for more sensitive and quantitative tools for the management of oc. Numerous analytes have been detected in saliva using various techniques table 2. Biomarkers in the saliva have the potential to be used for screening purposes in epidemiological studies. Currently, oral cancer diagnosis depends on a thorough oral examination, usually by a dentist or other qualified health care provider, for possible signs and symptoms of the disease. Salivary il8, il6 and tnf as potential diagnostic biomarkers for oral cancer haafsa arshad sahibzada 1, zohaib khurshid 2, rabia sannam khan 3, mustafa naseem 4, khalid mahmood siddique 5, maria mali 6 and muhammad sohail zafar 7,8 1 department of oral medicine, islamabad dental hospital idh, islamabad 44000, pakistan. Oral squamous cell carcinoma oscc accounts for more than 90% of all oral cancer.
Saliva is an easily accessible biofluid with immense diagnostic potential in oral cancer. The identification of potential saliva signatures for early, noninvasive detection of oral squamous cell carcinoma oscc lead to early detection, better outcome, and survival. This article aims to provide a brief overview of various salivary biomarkers and their implications in oral cancer. Saliva becomes the prime choice for screening and identification of biomarkers because of the fallen cancer cells in the oral cavity 126. Biomarkers are being used as diagnostic or monitoring tools for a wide variety of diseases, including systemic lupus erythematosus, sjogren disease, behcet disease. Lawrence,dds, msc, phd abstract because of interest in the link between oral. Previous studies have revealed the clinical efficiency of salivary biomarkers for oral cancers. Direct contact between saliva and the oral cancer lesion makes measurement of tumour markers in saliva an attractive alternative to serum testing. As overviewed in table 3, salivabased microbial biomarkers have also been linked to oral cancer and even obesity. A new approach for the diagnosis of systemic and oral. We describe the distinct features of the tongue, mucosa, and salivary glands.
Currently, oral cancer diagnosis depends on a thorough oral. Salivary proteomics for oral cancer biomarker discovery. Review article salivary rna signatures in oral cancer detection. Many of these constituents, their altered levels in oral cancer can serve as the potential biomarker and aid in its diagnosis. Pdf salivary analysis of oral cancer biomarkers iris. Roughly 90% of oral cancers histologically originate from squamous cells and are classified as oral squamous cell carcinoma oscc rivera, 2015. Over the past decade, there has been a dramatic increase in studies evaluating. Specific terms were used from january 1995 to march 2017 by three experts. This presentation provides an overview of the clinical applicability of salivabased biomarkers in oral cancer along with the discussion of research finding determining the clinical utility. Accepted papers reported at least 2 separate estimates. Despite progress in preventive and therapeutic strategies, delay in oscc diagnosis remains one of the major causes of high morbidity and mortality.
Introduction and application of biomarkers to oral cancer. Salivary biomarkers of stress can minimize some of the potential confounding variables associated with blood sampling 3. Nmr 1h and c based signatures of abnormal choline metabolism in oral squamous cell carcinoma with no prominent warburg effect. The combination of transcriptomic and proteo mic salivary markers is of great value for oral cancer detection and differentiation from pmod patients.
The salivary transcriptome coding, noncoding rnas has also displayed performance in multiethnic cohorts of oral cancer patients. More than 100 potential salivary biomarkers have been reported till date. In saliva, the tumorspecific dna was positive in 100% of the patients diagnosed with oral. Salivary cortisol level was used as an endocrine marker and salivary alphaamylase activity as. Dec 10, 2019 oral cancer is the sixth most common cancer type in the world, and 90% of it is represented by oral squamous cell carcinoma oscc. Pdf oral cancers are the sixth most frequent cancer with a high mortality rate. In addition to traditional tissue biopsybased diagnostics, more reliable, inexpensive, and noninvasive methods are required for early diagnosis of cancer. Salivary biomarkers for detection of oral squamous cell. Oral cancer has emerged as an alarming public health problem with increasing incidence and mortality rates all over the world. The existence of gene mutations can often be associated and used as biomarkers in order to diagnose oral cancer.1514 1212 504 1528 1423 1142 1071 899 1381 1143 438 1480 1502 660 725 223 1515 157 886 1560 659 248 1151 21 1188 1252 810 1036 640 1186 746 157 539 760 1562 1080 396 271 632 1010 1004 1363 931 502 399 412 367 863